By Doolittle E.
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Nacre from Haliotis laevigata was demineralized in acetic acid and subsequently remineralized with amorphous CaCO3 nanoparticles, which were formed in the presence of µg mL–1 amounts of polyaspartic acid . Via these amorphous precursors, we were able to completely ﬁll the compartments of the insoluble organic matrix with CaCO3 . Calcite was obtained instead of the natural aragonite and the single crystalline domains were smaller than for the natural archetype (Fig. 8). Nevertheless, the retrosynthesized nacre was indistinguishable from its natural archetype by electron microscopy (SEM and TEM) as shown in Fig.
Face-selective polymer adsorption can also lead to the formation of onedimensional particle aggregate arrays without the particle fusion of the individual nanoparticles that takes place during oriented attachment. This was demonstrated for silicalite nanoparticles, which self-assembled to chains after selective PDADMAC adsorption . Even very simple polymers without an obvious functionality can have an inﬂuence on a mineralization process, as demonstrated for the generation of porous silica in the presence of PEG .
The main acidic building blocks of GAGs are keratin sulfate, hyaluronic acid, chondroin-4-sulfate, chondroin-6-sulfate, dermatan sulfate, heparin sulfate, and heparin . Thus, polysaccharides are usually of importance when they covalently bind to proteins (glycoproteins for example in eggshell mineralization). They only rarely occur unbound as a soluble matrix in biomineralization and often have an alternating structure, usually of the AB copolymer type. They are therefore closer to the structure of polyelectrolytes than to that of proteins/peptides with multiple domain structures and functionalities.