New PDF release: Advanced Bacterial Genetics: Use of Transposons and Phage

By Kelly Thomas Hughes, Sidney P. Colowick, Nathan Oram Kaplan, Stanley R. Maloy

The seriously acclaimed laboratory common for greater than fifty years, equipment in Enzymology is without doubt one of the such a lot hugely revered guides within the box of biochemistry. for the reason that 1955, each one quantity has been eagerly awaited, often consulted, and praised via researchers and reviewers alike. Now with over four hundred volumes (all of them nonetheless in print), the sequence includes a lot fabric nonetheless appropriate today-truly an important book for researchers in all fields of lifestyles sciences. This new quantity offers tools concerning using bacterial genetics for genomic engineering. The publication comprises sections on pressure collections and genetic nomenclature; transposons; and phage.

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Additional info for Advanced Bacterial Genetics: Use of Transposons and Phage for Genomic Engineering

Sample text

5. Transposon insertions in operons are nearly always strongly polar on expression of downstream genes. Thus, transposon insertions can be used to determine whether genes are in an operon. 6. Transposons can generate deletions nearby. This provides a convenient method for isolating adjacent deletion mutations. 7. Transposons can provide a portable region of homology for genetic recombination. Transposon insertions can be used to construct deletions or duplications with defined endpoints, or can serve as sites of integration of other genetic elements.

And Lambowitz, A. (2002). ’’ ASM Press, Washington, DC. , and Botstein, D (1977). Genetic engineering in vivo using translocatable drug‐resistance elements. New methods in bacterial genetics. J. Mol. Biol. 116, 125–159. , and Gottesman, S. (1991). Uses of transposons with emphasis on Tn10. Methods Enzymol. 204, 139–180. [3] In Vivo Mutagenesis Using EZ‐Tn5TM By JOHN R. KIRBY Abstract Epicentre Biotechnologies has developed a suite of transposon‐based tools for use in modern bacterial genetics.

9. Transposon insertions can be obtained that are near but not within a gene of interest. Such insertions are useful for constructing defined deletions and duplications, as well as for genetic mapping. 10. Special transposons can be used to construct operon or gene fusions. , 2002). Transposase from the commonly used transposons Tn5 and Tn10 show clear DNA sequence specificity for the insertion site, but the site recognized is sufficiently permissive to allow the isolation of insertions in essentially any gene.

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