Read e-book online Analyzing Biomolecular Interactions by Mass Spectrometry PDF

By Jeroen Kool, Wilfried M. A. Niessen

This monograph studies all appropriate applied sciences in accordance with mass spectrometry which are used to review or reveal organic interactions generally.
prepared in 3 components, the textual content starts off through reviewing recommendations these days nearly thought of classical, corresponding to affinity chromatography and ultrafiltration, in addition to the newest concepts. the second one half focusses on all MS-based tools for the research of interactions of proteins with all periods of biomolecules. in addition to pull down-based ways, this part additionally emphasizes using ion mobility MS, capture-compound techniques, chemical proteomics and interactomics. The 3rd and ultimate half discusses different vital applied sciences often hired in interplay stories, reminiscent of biosensors and microarrays.
For pharmaceutical, analytical, protein, environmental and biochemists, in addition to these operating in pharmaceutical and analytical laboratories.

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Sample text

This process is sometimes indicated as laserinduced dissociation [92]. Obviously, the fragmentation of metastable ions can be induced by additional (high-energy) collisions in a collision cell, which may also result in CID. In MALDI-TOF-MS terms, the process of fragmentation of metastable ions is frequently called post-source decay (PSD) [93]. 6 LC–MSn in an ion-trap instrument: stepwise fragmentation of glucosylated Saponin 26. (Reprinted with permission from Ref. [89]. ) energy of these fragment ions md in PSD significantly differs from that of the parent ions mp , whereas their velocities are the same.

Surface-enhanced laser desorption ionization (SELDI-TOF-MS) is a variation of MALDI. Instead of an inert metal target, a modified target is applied to achieve biochemical affinity with the analyte molecules [33]. In SELDI, the protein mixture is spotted onto the surface with a specific (bio)chemical functionality, for example, cation- or anion-exchange materials, hydrophobic materials, or materials with immobilized metal affinity, lectin, or even protein or antibody affinity. Specific proteins in the mixture bind to the surface, while others can be removed by washing.

Crosstalk may occur on rapid switching between two selected-reaction monitoring (SRM, see below) transitions with the same product ion from two different precursor ions. Product ions of the first precursor ion are erroneously attributed to the second precursor ion, because these still reside in the collision cell while MS1 is already switched to the second precursor ion. A stacked-ring collision cell, featuring an axial traveling-wave or transient DC voltage to propel the ions and to reduce the transit times, has been reported as well [15].

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